Customizable T3 Elisa Test Kit for Accurate and Biomarker Quantification

The thyroid gland is essential for the development of many tissues and for the adjustement of their metabolism. These processes are under the control of thyroid hormones, T₄ and T₃.

Under stimulation by pituitary thyrotropin(TSH). the thyroid gland essentially secretes T₄ with minute amounts of T₃.

Tissue recptors to thyroid hormones are T₃ receptors, T₃ represents thus the acive entity among thyroid secretions.

The bulk of circulating T₃ is produced by peripheral deiodination of T₄ alternatively, T₄ yields reverse T₃(or RT₃), a poorly active thyronine.

In the blood,T₃ circulates free and bound to the same carrier protein transporting T₄, thyroxine binding globulin or TBG. Nevertheless, T₃ binding affinity to TBG is significantly less than that of T₄; The free T₃ serum concentration represents about 0.3% of the total circulating T₃ concentration.

Under normal conditions, total T₃ level is negatively. Correlated to serum TSH level due to the negative feed -back mechanism regulating thyroid funcion.

• This kit is designated for In-Vitro Diagnostic Use Only.
• Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with water and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
• Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
• Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.
• Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with