Anti-HCV Elisa Test Kit with High Specificity for 96 Tests and 1.5-3 Hours Assay Time

The Anti-HCV assay is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of HCV virus in human serum and plasma.

Hepatitis C virus is the most common cause of posttransfusion and community-acquired non-A, non-B hepatitis worldwide. Infection with HCV frequently leads to chronic hepatitis and cirrhosis, and is associated with the development of hepatocellular carcinoma. Hepatitis C virus is an enveloped, positive-sense single-stranded RNA virus which has been classified as an own genus in the family of Flaviviridae. The genome consists of approximately 9.5 kb encoding for a 3000 amino acid polypeptide of structural and non-structural domains. Like other RNA viruses, the HCV genome exhibits substantial heterogeneity as a result of mutations that occur during viral replication. Worldwide, at least 11 genetically distinct genotypes and multiple subtypes and virus variants have been described. Infection with specific genotypes can affect disease severity and treatment response. Hepatitis C is primarily transmitted through contaminated blood and blood products and to a lower extent by human body secretions.

Indirect method. Total duration of assay: 100 minutes

The Anti-HCV ELISA employs solid phase, indirect ELISA method for detection of antibodies to HCV in two-step incubation procedure. Polystyrene microwell strips are pre-coated with recombinant, highly immunoreactive antigens corresponding to the core and the non-structural regions of HCV (NS3, NS4 and NS5), that can detect all HCV genotypes. During the first incubation step, Anti-HCV specific antibodies, if present, will be bound to the solid phase pre-coated HCV antigens. The wells are washed to remove unbound serum proteins, and mouse anti-human IgG antibodies (anti-IgG) conjugated to the enzyme horseradish peroxidase (HRP-Conjugate) are added. During the second incubation step, these HRP-conjugated antibodies will be bound to any antigen-antibody(IgG) complexes previously formed and the unbound HRP-conjugate is then removed by washing. Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added to the wells and in presence of the antigen-antibody-anti-IgG (HRP) immunocomplex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and it is proportional to the amount of antibody captured in the wells, and to the amount of antibody in the sample respectively. Wells containing samples negative for Anti-HCV remain colorless.